PE-Designer

A pegRNA designer for CRISPR prime editing.

Prime editing tools that consist of a reverse transcriptase linked with Cas9 nickase are capable of generating targeted insertions, deletions, and base conversions without producing DNA double strand breaks or requiring any donor DNA.

Citation Hwang G.H. et al. PE-Designer and PE-Analyzer: web-based design and analysis tools for CRISPR prime editing. Nucleic Acids Research, gkab319 (2021).

Result will be notified by e-mail if provided.

PAM Type

52 PAM types available

Target Genome

Genomes

159 assemblies across 84 species

The dark block is what the pegRNA has to write; red residues are the ones it changes.

K R P C N X S I S A A S K H A F V G L M L F F C F S S P G L G Q S L G P R L S T X W Q R K G S P A S S R G R R R T A F P R Q G L V C A A P A P G Y W L T S L A
AAACGCCCATGCAATTAGTCTATTTCTGCTGCAAGTAAGCATGCATTTGTAGGCTTGATGCTTTTTTTCTGCTTCTCCAGCCCTGGCCTGGGTCAATCCTTGGGGCCCAGACTGAGCACG---TGATGGCAGAGGAAAGGAAGCCCTGCTTCCTCCAGAGGGCGTCGCAGGACAGCTTTTCCTAGACAGGGGCTAGTATGTGCAGCTCCTGCACCGGGATACTGGTTGACAAGTTTGGCTGG
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AAACGCCCATGCAATTAGTCTATTTCTGCTGCAAGTAAGCATGCATTTGTAGGCTTGATGCTTTTTTTCTGCTTCTCCAGCCCTGGCCTGGGTCAATCCTTGGGGCCCAGACTGAGCACGCTTTGATGGCAGAGGAAAGGAAGCCCTGCTTCCTCCAGAGGGCGTCGCAGGACAGCTTTTCCTAGACAGGGGCTAGTATGTGCAGCTCCTGCACCGGGATACTGGTTGACAAGTTTGGCTGG
K R P C N X S I S A A S K H A F V G L M L F F C F S S P G L G Q S L G P R L S T L X W Q R K G S P A S S R G R R R T A F P R Q G L V C A A P A P G Y W L T S L A

Reference sequence

The region as it reads now, or FASTA with both records.

FASTA, or a bare sequence. Several records are designed separately and reported separately.

Edited sequence

The same region as it should read after the edit. The difference between the two is what the pegRNA has to write.

Leave empty only if the reference box holds FASTA with both records in it. Leave both boxes empty to run the example shown.