Cas-Designer takes a target sequence and lists every guide RNA that can be placed in it for the chosen nuclease, then ranks the candidates so the most promising ones are easy to spot. Each candidate is reported with its GC content, its out-of-frame score, and the number of potential off-target sites found in the selected genome.
Result will be notified by e-mail if provided.
52 PAM types available
Genomes
159 assemblies across 84 species
Guides are extracted from your sequence and off-targets are searched with cas-offinder.
Usage: [start_position1]-[end_position1];[start_position2]-[end_position2];…
Example: 10-30;34-39;48-60