Guide RNA design, variant-aware off-target search, and editing-outcome analysis — one connected workflow, no account required.
Find and rank candidate gRNAs in a target sequence — GC content, out-of-frame score and off-target counts side by side.
Search a reference genome for potential off-target sites across 52 PAM types, with mismatch and DNA/RNA bulge tolerance.
Haplotype-aware off-target search that folds an individual’s variants from a VCF into the reference before matching.
Quantify indel frequencies and editing efficiency at a target site directly from paired-end NGS reads.
Browse precomputed guide RNA and off-target sets across the supported genome assemblies.