From guide
design to
editing outcome
— in one place.

Computational tools and libraries for RNA-guided genome editors.

Available tools

Off-target

Find where else a guide could cut — across a reference genome, or in one person’s.

Variant-aware Cas-OFFinder Off-target search · VCF

Incorporate personal VCF variants to find variant-specific off-targets.

Open Cas-OFFinder 2 Off-target search

Search genome-wide potential off-target sites for any PAM and nuclease.

Open

Design

Pick and rank guide RNAs for a target sequence.

Cas-Designer Guide RNA design

Design optimal guide RNAs with on-target scoring for your target sequence.

Open

Analysis

Quantify what an edit actually did, from sequencing reads.

Cas-Analyzer Editing outcome analysis

Quantify insertions, deletions, and HDR from your sequencing reads.

Open CReVIS-seq Integration site analysis

Find where a lentivirus integrated, from the junction between its LTR and the genome.

Open CRISPR-Sub Editing outcome analysis

Detect substitutions Cas9 left behind by comparing a treated sample with a mock.

Open

Database

Look up guides and off-targets that are already computed.

Cas-Database Reference data

Browse precomputed SpCas9 (NGG) guide RNAs and off-targets across supported species.

Open Cpf1-Database Reference data

Browse precomputed Cas12a/Cpf1 (TTTV) guide RNAs and off-targets across supported species.

Open

Base editing

Design base editing guides, then measure what they converted.

BE-Designer Base editing · design

Find guides whose editing window carries the base you want to convert.

Open BE-Analyzer Editing outcome analysis

Quantify base conversions, indels and editing windows from your sequencing reads.

Open

Prime editing

Design pegRNAs for a precise edit.

PE-Designer Prime editing · design

Find prime editing targets from the sequence you have and the one you want.

Open PE-Analyzer Editing outcome analysis

Quantify prime edits, partial edits and unintended indels from your sequencing reads.

Open