BE-Designer

Guide RNAs whose editing window carries the base you want to change

A base editor does not cut — it converts one base into another inside a narrow window a fixed distance from the PAM. That makes guide selection a different question from nuclease work: a guide is only useful if the base you want to change actually falls inside that window. BE-Designer takes a target sequence and an editor, finds every protospacer whose window carries the base that editor converts, and discards the rest.

Citation Hwang G-H. et al. Web-based design and analysis tools for CRISPR base editing. BMC Bioinformatics 19, 542 (2018).

Result will be notified by e-mail if provided.

PAM Type

52 PAM types available

Target Genome

Genomes

159 assemblies across 84 species

Target sequence

FASTA, or a bare sequence. Several records are designed separately and reported separately. Leave it empty to run the example shown.

How much of each target is the protospacer, PAM excluded.

Base editor

to

Counted from the PAM — position 1 is the base next to it.

Only guides whose window are reported — a guide the editor cannot act on is not a candidate.

A cytidine or adenine deaminase fused to dCas9, converting C to T or A to G within the editing window of the sgRNA-bound protospacer